International Scholarly Research Notices

Research Article

Evaluation of Viability and Proliferation Profiles on Macrophages Treated with Silica Nanoparticles In Vitro via Plate-Based, Flow Cytometry, and Coulter Counter Assays

Table 1

Assays used to determine cell viability and proliferation in RAW 264.7 cells exposed to SiO2 NP (7 nm) in vitro.
AssayReadoutInstrumentControls used
CellTiter-blue cell viability assay (Promega)Mitochondrial activityPlate reader Untreated; no cell control; pre- and postspike controls; positive control
In vitro toxicology assay kit XTT based (Sigma)Mitochondrial activity
CytoTox-one homegenouse membrane integrity assay (Promega)Membrane integrity
CyQuant NF cell proliferation assay (Invitrogen)Proliferation
PI (Invitrogen)Membrane integrityFlow cytometer Untreated; positive control
Calcein am stain (Invitrogen)Intracellular esterase activity
CFSE (Invitrogen)Proliferation/cell tracking
Coulter counterCell number and sizeCoulter counterUntreated; LPS control