Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage
Figure 4
Morphological dimensions of sorted satellite cells. (a) Maximum width was recorded from several random fields for each subpopulation of cells, at 2, 4, and 7 days after culture in 20% serum. The number of cells measured for each subpopulation at each day is indicated below each bar. Statistical differences between data points are represented by lines (). The asterisk indicates statistically significant difference with all other data points (). (b) Aspect ratio length : width measured from the four subpopulations at days 4 and 7 in culture. The number of cells for each condition is indicated below each bar. The populations expressing α2/δ1+ at the time of sorting had the largest aspect ratios of the four groups at day 4, and the ratio was even larger for the α2δ1+/CD34− group at day 7. Statistical differences between data points are represented by lines (). The discontinuous line with an asterisk represents statistically significant difference between α2δ1- cells and α2δ1+ cells ().