Research Article

Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage

Figure 4

Morphological dimensions of sorted satellite cells. (a) Maximum width was recorded from several random fields for each subpopulation of cells, at 2, 4, and 7 days after culture in 20% serum. The number of cells measured for each subpopulation at each day is indicated below each bar. Statistical differences between data points are represented by lines ( ). The asterisk indicates statistically significant difference with all other data points ( ). (b) Aspect ratio length : width measured from the four subpopulations at days 4 and 7 in culture. The number of cells for each condition is indicated below each bar. The populations expressing α2/δ1+ at the time of sorting had the largest aspect ratios of the four groups at day 4, and the ratio was even larger for the α2δ1+/CD34 group at day 7. Statistical differences between data points are represented by lines ( ). The discontinuous line with an asterisk represents statistically significant difference between α2δ1- cells and α2δ1+ cells ( ).
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(a)
460842.fig.004b
(b)