Elimination of the Actin-Binding Domain in Kelch-Like 1 Protein Induces T-Type Calcium Channel Modulation Only in the Presence of Action Potential Waveforms
Figure 6
Changes in the repolarization slope in conjunction with an afterhyperpolarization slope synergize to enhance Ca2+ influx by ΔKelch. (a) Protocols with a sloping afterhyperpolarization ramp of 0.75 mV/ms were combined with repolarizing slopes of 70 or 35 mV/ms (blue and orange, resp.), and compared with a waveform with instantaneous repolarization (black). (b) Percentage of increase in calcium influx with the three waveforms with respect to control; the magnitude of the changes induced by the original APWs is shown with dotted lines (; ); .