5.0% Tricine ND-PAGE and ND-zymogram analysis containing 0.15% xylan, using crude xylanase preparations from strain TWXYL3, depicting the presence of high-molecular-weight proteins and high-molecular-weight active xylanases. Molecular mass markers are Precision Plus Protein Standards (Bio-Rad). (a) ND-PAGE analysis of crude enzyme preparations (50, 25, and 10 μg loading), stained with CBB. (b) Zymogram analysis of high-molecular-weight crude xylanase enzymes (50, 25, and 10 μg loading). Xylanase activity is indicated by zones of clearing. (c) A 7.5% Tricine SDS-PAGE zymogram containing 0.15% oat spelt xylan as substrate, using crude xylanase preparation (50 μg) from strain TWXYL3. Xylanase activity is indicated by zones of clearing.