Journal of Amino Acids

Research Article

Elucidation of the Rotavirus NSP4-Caveolin-1 and -Cholesterol Interactions Using Synthetic Peptides

Table 5

Treatment with cholesterol-altering drugsa.
InhibitorActionResults
FillipinCholesterol-binding; disrupts caveolae and caveolar membrane transport.Disrupted NSP4 transport
NystatinCholesterol-binding; disrupts caveolae and caveolar membrane transport.Disrupted NSP4 transport
ChlorpromazineInhibits clathrin-dependent endocytosis and coated pit processes.Normal NSP4 transport to the PM.
NocodazoleDisrupts microtubule polymerizationDisrupted NSP4 transport
aEach inhibitor was reacted with RV-infected MDCK cells. The cells were washed, stained for NSP4 (anti-NSP4150-175) and a fluorescent secondary antibody (rabbit anti-IgG-FITC), and the intracellular location compared to untreated, infected cells. Chlorpromazine and nocodazole were included as controls.