Development of Safer Gene Delivery Systems to Minimize the Risk of Insertional Mutagenesis-Related Malignancies: A Critical Issue for the Field of Gene Therapy
Figure 5
Self-inactivating retroviridae-based vector systems can be engineered via deletion of the viral enhancer/promoter U3 region in the 3′-LTR (upper part of the figure). This deletion ultimately results in a proviral form with two nontranscribing LTRs in transduced cells (lower part of the figure). At this point, only the internal promoter is able to transcribe the mRNA encoding for the transgene.
