Chromatin-Associated Proteins Revealed by SILAC-Proteomic Analysis Exhibit a High Likelihood of Requirement for Growth Fitness under DNA Damage Stress
Figure 1
Heavy lysine is efficiently incorporated in fission yeast. (a) A schematic diagram shows the consecutive subculturing of cells in heavy lysine containing medium. (b) An MS spectrum of the Eno1 peptide AVGNVNNIIAPVVK. The spectrum of the peptide resulted from passages p0, p1, p2, and p3 is shown. Green and red lines indicate the m/z of light and heavy peptides, respectively. Arrow indicates the position of light peptide undetected in p2 and p3 cells. (c) The scatter plot shows the ratio of light and heavy peptides in the first passage (sample p1). X- and Y-axis indicate the abundance of individual peptides and the ratio of light and heavy peptide, respectively. Each dot represents a peptide.
