Western blot analysis of expression for vimentin and E-cadherin proteins in whole cell lysates. Up-regulation of vimentin protein was evidently demonstrated in HN008 and HN5 cell lines, while E-cadherin protein was down-regulated in all five oral cancer cell lines in comparison with a normal oral keratinocyte cell line in the presence or absence of stimulation with 10 μg/mL of the cell wall extract of Fusobacterium nucleatum (FnCW), a gram-negative periodontal bacterium, or 10 ng/mL of PMA (a phorbol ester and epithelial activator) overnight. Expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), serving as a housekeeping gene control, was approximately equivalent among different samples, indicating equal loadings. The molecular weight of each protein was as predicted.