The distribution of cytosolic proteins in reversed-phase chromatographic fractions of 0.15 M (a), 0.25 M (b), 0.4 M (c), and 1.0 M (d) potassium chloride anion-exchange fractions, derived from a single DDF extract of cultured human Neuro-2A cells. Proteins were quantitated using a BCA method and quantities were normalised as a percentage of total extracted protein (top). Samples of all fractions were analysed using SDS-PAGE stained with Coomassie Blue G-250 (bottom) and total cytosolic extract is also shown (1) for comparison. It is evident that proteins from each of the anion-exchange fractions eluted over a broad range of hydrophobicity using the reversed-phase protocol and were therefore further fractionated.