An Economical High-Throughput Protocol for Multidimensional Fractionation of Proteins
Figure 5
Representative comparative Coomassie Blue G250-stained analytical SDS-PAGE analyses of four MDLC fractions. These are in all cases the 45% (v/v) acetonitrile reversed-phase chromatographic fractions of 0.15 M (a), 0.25 M (b), 0.4 M (c), and 1.0 M (d) potassium chloride anion-exchange fractions, originally derived from cytosolic DDF extracts of twelve samples of cultured human Neuro-2A cells.