No angiogenic response in ischemic hind limbs of db1 and Lepr^db/db mice after adenovirus-mediated gene transfer of adiponectin. (a) Adenoviral vectors expressing adiponectin or β-galactosidase (control) were delivered intravenously via the jugular vein at 3 days prior to surgery. Quantitative analysis of ischemic/nonischemic LDBF ratio in WT, db1, and Lepr^db/db mice without adenovirus-mediated gene transfer (= control) or with adenovirus-mediated gene transfer (Ad-βgal and Ad-APN) on postoperative day 14. * versus control of WT, db1, and Lepr^db/db mice without adenovirus-mediated gene transfer. (b) Quantitative analysis of capillary density in WT, db1 and Lepr^db/db mice in ischemic adductor muscle on postoperative day 14 () without adenovirus-mediated gene transfer or with adenovirus-mediated gene transfer (Ad-βgal and Ad-APN). Capillary density was expressed as the number of capillaries per high-power field (×400). .