Variable Resistance of RMS to Interferon γ Signaling
Figure 3
RMS cells show intact IFNRs and STAT1 phosphorylation in vitro. (a, b, and c) Flow cytometry data of IFNGR subunit expression in RMS and HT29 control cells lines; filled histograms represent expression levels using specific antibodies; open histograms represent isotype control stainings; (d) mutation analysis of STAT1 phosphorylation site and JAK binding motif in RMS cell lines and HT29 control cells; (e) western blot analysis of p STAT1 induction 24 h and 48 h after IFNγ treatment; β-actin serves as loading control.