Effects of Somatic Mutations in the C-Terminus of Insulin-Like Growth Factor 1 Receptor on Activity and Signaling
Figure 1
Cellular autophosphorylation activity of wild-type and mutant receptors. R-cells were transfected with wild-type or mutant forms of IGF1R. After overnight starvation, cells were treated with or without IGF1 for 10 minutes. (a) Top panel: IGF1R was immunoprecipitated and analyzed by anti-pY1135/pY1136 Western blotting. Bottom panel: the membrane was stripped and reprobed with anti-IGF1R antibody. (b) Densitometric analysis was carried out, and the pY1135/pY1136 signal was normalized to the total IGF1R signal. The bar graph shows results from three experiments (mean ± standard deviation). The results were analyzed by Student’s t-test, and no statistically significant differences were observed between wild type and mutants (P > 0.1).
