Effects of Somatic Mutations in the C-Terminus of Insulin-Like Growth Factor 1 Receptor on Activity and Signaling
Figure 3
Peptide phosphorylation by purified wild-type and mutant cytoplasmic domains. The preautophosphorylated proteins (800 nM) were incubated with 1 mM peptide (KKEEEEYMMMMG) and 1 mM ATP, and activity was measured using the phosphocellulose paper binding assay. Reactions proceeded for 20 minutes at 30°C. Assays were conducted in triplicate, and the results are presented as mean ± standard deviation
