Effects of Somatic Mutations in the C-Terminus of Insulin-Like Growth Factor 1 Receptor on Activity and Signaling
Figure 4
IRS-1 phosphorylation. R-cells were transfected with wild-type or mutant forms of IGF1R. After overnight starvation, cells were treated with or without IGF1 for 10 minutes. Endogenous IRS-1 was immunoprecipitated and analyzed by antiphosphotyrosine Western blotting. The membrane was stripped and reprobed with anti-IRS-1 antibody.
