Effects of Somatic Mutations in the C-Terminus of Insulin-Like Growth Factor 1 Receptor on Activity and Signaling
Figure 7
MAP kinase activation. R-cells were transfected with the indicated forms of IGF1R. After starvation and treatment with IGF1, MAP kinase activity was measured in cell lysates using a phosphospecific antibody. Membranes were stripped and reprobed with anti-MAPK antibody. The bar graph shows the ratio of phospho-MAPK to total MAPK (mean ± standard deviation). The fold increases in the presence of IGF1 are given below the bar graph. A representative phospho-MAPK blot is shown above the graph. The results were analyzed by Student’s t-test, and no statistically significant differences were observed between wild type and mutants (P > 0.1).
