Research Article
Dried Blood Spot Sampling with LC-MS Analysis for Routine Therapeutic Caffeine Monitoring in Neonates
Table 1
Intraday and interday accuracy (RE%) and precision (CV%) results for the LC-MS caffeine DBS method
.
| | | Nominal conc. (ng/mL) | Intraday | | | 500 | 1000 | 5000 | 8000 | 15000 | 20000 | 25000 |
| | run 1 | Mean conc. (ng/mL) | 441.8 | 1034.2 | 5346.7 | 8325.0 | 15138.9 | 20061.8 | 24087.7 | | SD | 75.0 | 20.2 | 698.2 | 339.6 | 668.4 | 1668.9 | 140.1 | | RE% | −11.8 | 3.2 | 6.8 | 3.9 | 0.8 | 0.1 | −3.8 | | CV% | 17.0 | 2.0 | 13.1 | 4.1 | 4.4 | 8.3 | 4.7 |
| | run 2 | Mean conc. (ng/mL) | 562.0 | 1113.4 | 5237.2 | 7820.4 | 15204.0 | 17404.8 | 21902.6 | | SD | 38.3 | 92.9 | 182.4 | 202.2 | 2112.4 | 604.3 | 1519.3 | | RE% | 12.4 | 11.3 | 4.7 | −2.2 | 1.4 | −13.0 | −12.4 | | CV% | 6.8 | 8.3 | 3.5 | 2.6 | 13.9 | 3.5 | 6.9 |
| | run 3 | Mean conc. (ng/mL) | 503.6 | 971.5 | 4970.3 | 7387.8 | 15702.9 | 21355.2 | 24908.2 | | SD | 60.1 | 38.9 | 341.1 | 318.0 | 1466.4 | 1766.7 | 2160.4 | | RE% | 0.7 | −2.8 | −0.6 | −7.7 | 4.7 | 6.8 | −0.4 | | CV% | 12.4 | 4.0 | 6.9 | 4.3 | 9.3 | 8.3 | 8.7 |
| | | | Interday |
| | | Mean conc. (ng/mL)
| 502.4
| 1039.7
| 5184.7
| 7844.4
| 15348.6 | 19607.3 | 23632.8 | | | SD | 75.1 | 77.6 | 456.9 | 469.6 | 1485.7 | 2165.5 | 2070.2 | | | Average RE% | 0.4 | 3.9 | 3.6 | −2.0 | 2.3 | −2.0 | −5.5 | | | Overall CV% | 14.9 | 7.5 | 8.8 | 6.0 | 9.7 | 11.0 | 8.8 |
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