Influenza Research and Treatment

Research Article

Genetic Analysis of Avian Influenza Viruses: Cocirculation of Avian Influenza Viruses with Allele A and B Nonstructural Gene in Northern Pintail (Anas acuta) Ducks Wintering in Japan

Table 1

Avian influenza viruses used in the present studya.
VirusGene segment sequencedHA0 cleavage site
(*cleavage point)b
A/pintail/Aomori/422/07 H1N1HA, NP, NA, and MPSVQSR*GLF
A/pintail/Aomori/794/08 H1N1HA, NP, NA, and MPSIQSR*GLF
A/pintail/Miyagi/1472/08 H1N1HA, NP, NA, M, and NSPSIQSR*GLF
A/pintail/Akita/1265/08 H1N2HA, NP, NA, M, and NSPSIQSR*GLF
A/pintail/Akita/1353/08 H1N2HA, NP, NA, M, and NSPSIQSR*GLF
A/pintail/Akita/1364/08 H1N2HAc, NP, NA, M, and NSPSIQSR*GLF
A/pintail/Aomori/1130/08 H1N3PB2, PB1c, PA, HA, NP, NA, M, and NSPSIQSR*GLF
A/pintail/Aomori/1192/08 H5N9PA, NP, M, and NS—d
A/pintail/Akita/1256/07 H5N2NS—
A/pintail/Akita/1355/07 H5N2NS—
A/pintail/Aomori/385/08 H5N3NS—
A/pintail/Aomori/372/08 H7N7NS—
A/pintail/Miyagi/674/08 H7N7NS—
aThe viruses were isolated from apparently healthy migratory pintails wintering in Japan. Viruses were isolated in embryonated chicken eggs. The entire open reading frame (until otherwise mentioned) of each gene segment was sequenced from PCR product; bthe amino acid sequences were deduced from nucleotide sequences; cpartial sequences—for H1 HA 765 base pair (760-1524) and for PB1 1350 base pair (1-1350); dnot applicable.