Mycophenolic Acid Overcomes Imatinib and Nilotinib Resistance of Chronic Myeloid Leukemia Cells by Apoptosis or a Senescent-Like Cell Cycle Arrest
Figure 2
MPA induced a moderated apoptosis. K562-S, K562-R, and K562-RN cells (2.105/mL) were grown in the presence of vehicle only, MPA (3 μg/mL) or incubated with Z-vad-fmk (50 μM) and MPA (3 μg/mL) for 3 days. An aliquot was incubated for 15 min in the presence of annexin-V and propidium iodide. Samples were analyzed by flow cytometry and labelled cells were analyzed as described in Section 2. Results from 6 experiments are expressed as the % of annexin V-labelled cells in comparison to a positive control corresponding to imatinib-treated K562 cells ((a), n = 6). Samples treated as above were used for detection of the cleaved form of caspase 3 by Western blot and Hsp60 (as loading control) (b).
