Ingenuity Pathways Analysis of misregulated proteins from alcohol-treated whole-embryo culture. Proteins with altered regulation were analyzed with Ingenuity Pathways Analysis online software. This Core Analysis highlights the signaling and metabolic pathways, molecular networks, and biological processes that are most significantly perturbed in the alcohol-treated cultures. The Ingenuity Path Designer feature was utilized to abridge the molecular network. The proteins are represented by shapes that reflect protein function (https://analysis.ingenuity.com/pa/info/help/help.htm#legend.htm) and labeled with their gene name. Proteins identified in alcohol-treated embryos have shaded shapes; molecules not identified in this study have clear shapes. These molecules were included in the abridged network if they were linked to multiple identified proteins or formed integral links between identified proteins. Solid lines indicate direct interactions and dashed lines are indirect interactions. Abbreviations: Actg1—Gamma actin; Cdkn2a—Cyclin-dependent kinase inhibitor 2A; Ifng—Interferon gamma; Ikbke—Inhibitor of kappa b kinase epsilon; Krt72—Keratin 72; Myc —v-myc myelocytomatosis viral oncogene homolog (avian); Pabpc1—poly(A) binding protein, cytoplasmic 1; Pcbp1—poly(rC) binding protein 1; Psmb7—Proteasome subunit β type 7; Psmd6—Proteasome 26S subunit, non-ATPase, 6; St13— Hsp70 interacting protein; Tagln2—Transgelin 2; Trf (Tf)—Serotransferrin; Tpi1—Triosephosphate isomerase 1; Ube2n—Ubiquitin-conjugating enzyme E2N.