Biomarker Discovery of Pancreatic and Gastrointestinal Cancer by 2DICAL: 2-Dimensional Image-Converted Analysis of Liquid Chromatography and Mass Spectrometry
Figure 1
Discovery and validation of prolyl-hydroxylated α-fibrinogen as a pancreatic cancer biomarker (partially changed from [5]). (a) 2DICAL images of the peak (m/z, 827; RT, 8.3 min) with coordinates RT versus patients (upper) and intensity versus patients (lower). Red indicates samples from pancreatic cancer patients, and blue indicates samples from healthy controls. (b) Western blot of prolyl-hydroxylated α-fibrinogen (upper panel) and total α-fibrinogen (lower panel). (c) Large-scale ELISA validation of the plasma level of prolyl-hydroxylated α-fibrinogen using hundreds of clinical samples.
