Antigen presentation and TEa differentiation in tumor draining lymph node (DLN) with LPS injection. Mice were adoptively transferred with TEa cells and 24 h later injected with B16EaRed cells or PBS. On day 5, some mice received intratumoral LPS injection and 48 h later the DLN were excised and analyzed by FACS. (a) Mean absolute number of YAe⁺, CD86⁺ cells in DLN (gated on CD11c⁺, B220⁻ cells). (b) Dot plot showing CD11c⁺, B220⁻ cells. (c) Absolute numbers of INF- producing cells (gated on CD4⁺, CD90.1⁺ cells). (d) Dot plot showing CD4⁺, CD90.1⁺ cells. (e) Dot plot showing cells CD4⁺, CD90.1⁺ IFNγ ⁺ cells. Numbers in the plots represent mean percentages in gates. Data representative of two different experiments, mice per group. ().