Research Article
Methylenetetrahydrofolate Reductase C677T and A1298C Mutations in Women with Recurrent Spontaneous Abortions in the Northwest of Iran
Table 2
Primer pairs used for screening of MTHFR mutations by ARMS-PCR.
| Mutation
| Forward primer | Reverse primer | PCR | | product (bp) |
| | MTHFR | 5-TGC TGT TGG AAG GTG CAA GAT-3
| RW 5-GCG TGA TGA TGA AAT CGG-3 | 226 | C677T
| RM 5-GCG TGA TGA TGA AAT CGA-3 | 226 |
| | MTHFR | 5 -CCTTTGGGGAGCTGAAGGACTACTAC-3
| RW 5-CAAAGGACTTCAAAGACAGTC-3 | 120 | A1298C
| RM 5-GGTAAAGAACAAAGACTTCAAAGACACTGTG-3 | 127 |
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