Glycosaminoglycans Modify Elastase Action In Vitro and Enhance Elastase-Induced Cell Death in Cultured Fibroblasts
Figure 2
Effect of GAGs on HNE circular dichroism spectra. Spectra of HNE (3.9 μM) in absence or presence of GAGs (134 μM) were determined in 200 mM Tris buffer pH 8.0, at 260–200 nm and 36°C, as described in Section 2. Percentage values of secondary structures are shown in Table 2. HS and specially C6S changed the HNE secondary structure. HS: heparan sulfate; C6S: chondroitin 6-sulfate.
