Effect of GAGs on membrane integrity of NCM-exposed fibroblasts. Cells were incubated with NCM (HNE activity of 8.0 or 15 nM), in the absence or presence of C6S or HS (10 μM), for 16 h in the cell culture conditions. After this period, cells were collected by trypsinization, stained by PI, and analyzed by flow cytometry. A total of 10,000 events were registered. (a) statistically differs from Control (untreated cells); (b) differs from NCM and HS groups; (c) differs from NCM and C6S groups; (d) differs from HNE group ().