Research Article
Glycosaminoglycans Modify Elastase Action In Vitro and Enhance Elastase-Induced Cell Death in Cultured Fibroblasts
Table 1
Effect of GAGs on the hydrolysis of MeOSuc-Ala-Ala-Pro-Val-pNA by HNE.
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HNE (6.0 nM) in 200 mM Tris buffer pH 8.0 was preincubated for 5 min at 37°C with or without GAGs (300 nM). After that, MeOSuc-Ala-Ala-Pro-Val-pNA (0.10 to 2.5 mM) was added, and the hydrolysis was followed by the absorbance in 405 nm. Hep: heparin; HS: heparan sulfate; KS: keratan sulfate; C4S: chondroitin 4-sulfate; C6S: chondroitin 6-sulfate; DS: dermatan sulfate. |