The CD14+ monocytes subpopulation is permissive to infection with DENV-2. PBMCs were infected with DENV-2 at an MOI of 0.1 for 24 hours. Significant differences were observed only in infected CD14+ monocytes compared to the mock (a). A representative result from one of the healthy subjects is shown, in whom it can be seen that only CD14+ monocytes have a higher percentage of DENV positive cells compared to mock (1.4% versus 0.5%) (b). Results expressed as box and whisker plots summarizing data from eight healthy subjects. Significance was calculated using the Mann-Whitney test  .