DENV-2 induces the production of proinflammatory cytokines in PBMCs. PBMCs were infected with DENV-2 at an MOI of 0.1 for 6, 12, or 24 hours; concentrations of TNF-α (a) and IL-6 (b) were quantified in the supernatants using a CBA assay. The data were obtained after subtracting the concentration values of each cytokine quantified in the supernatants of the mock-inoculated cells. The median values obtained from mock-inoculated cells were 4 pg/mL (range, 0–14) and 125 pg/mL (range, 56–1153) for TNF-α and IL-6, respectively. Results expressed as box and whisker plots summarizing data from three healthy subjects.