Treatment with rh-sST2 decreases the production of TNF-α and IL-6 in human CD14+ monocytes stimulated with LPS, but not in DENV-2-infected monocytes. Monocytes were pre-treated with 10 μg/mL of rh-sST2 or IgG for 3 hours and were then stimulated or not with LPS (a) or were pre-treated with 10, 25, or 50 μg/mL of rh-sST2 or IgG for 3 hours and were infected or not with DENV-2 using an MOI of 0.1 ((b) and (c)) for 12 hours, at which time TNF-α and IL-6 were quantified in the culture supernatants using a CBA assay. The data were obtained after subtracting the concentration values of each cytokine quantified in the supernatants of the mock-inoculated cells. The median values obtained from mock-inoculated cells were 25 pg/mL (range, 9–49) and 251 pg/mL (range, 202–1104) for TNF-α and IL-6, respectively. Results expressed as box and whisker plots summarizing data from six healthy subjects. Significance was calculated using the Wilcoxon test  .