Adipogenic differentiation: Oil Red O staining. Oil Red O staining shows that the cells were able to differentiate towards the adipogenic lineage. Cells of the control group (Sham = (a)) and cells of animals of the OVX + Diet (b) group showed an obvious adipogenic differentiation. Cells of OVX + Steroid (c) animals had a rather weak adipogenic differentiation potential. Cells cultivated in the control medium showed no fat vacuoles stained by Oil Red O ((d) = Sham, (e) = OVX + Diet, and (f) = OVX + Steroid).