Quantitative real-time PCR of osteogenic differentiation markers BSP, OC, and runx2. The figure shows the expression levels of bone sialoprotein (BSP), osteocalcin (OC), and Runt-related transcription factor 2 (runx2) of cells of all groups (Sham; OVX + Diet; OVX + Steroid), comparing the expression levels of the cells grown in the osteogenic differentiation medium (OD) or in the control medium (NC).