Research Article

Profiling of Phytochemicals in Tissues from Sclerocarya birrea by HPLC-MS and Their Link with Antioxidant Activity

Table 2

Total phenol, FRAP, and DPPH radical-scavenging activities for various leaf, bark, and root crude and dialysed extracts of marula tree (S. birrea). Values are expressed as means ± standard deviations ( = 3)1.

ExtractTPC (GAE g/100 g DWS)FRAP (TE g/100 g DWS)DPPH IC50 (μg/mL)DPPH (TE g/100 g DWS)

Leaf hexane 10.00 ± 0.01a0.08 ± 0.03a176.57 ± 1.42e4.27 ± 0.34a
Leaf ethylacetate18.29 ± 0.02b8.02 ± 0.76b76.63 ± 5.31d9.82 ± 0.69a
Leaf <3.5 kDa53.88 ± 0.02e72.84 ± 1.88e8.42 ± 0.36ab 89.20 ± 3.95e
Leaf >3.5 kDa44.53 ± 0.01d55.10 ± 2.23d13.88 ± 0.40b 54.07 ± 1.80c
Leaf Water 45.35 ± 0.001d56.10 ± 2.20d12.23 ± 0.24b61.36 ± 1.19c

Bark hexane 48.43 ± 0.02de88.12 ± 8.09f9.91 ± 0.59ab75.85 ± 4.59d
Bark ethylacetate 23.02 ± 0.01bc23.33 ± 1.19c26.12 ± 3.09c28.30 ± 3.20b
Bark <3.5 kDa100.17 ± 0.01i216.64 ± 8.40k5.11 ± 0.10a146.86 ± 2.72i
Bark >3.5 kDa69.66 ± 0.003f102.46 ± 5.49g5.95 ± 0.50ab126.64 ± 10.77g
Bark water90.33 ± 0.03h197.34 ± 5.58j4.08 ± 0.16a183.97 ± 7.03j

Root hexane 26.53 ± 0.005c26.63 ± 3.49c29.65 ± 0.21c25.39 ± 1.85b
Root ethylacetate78.64 ± 0.02g114.59 ± 5.31h6.23 ± 0.20ab120.38 ± 4.01g
Root <3.5 kDa101.59 ± 0.01i220.41 ± 4.65k5.44 ± 0.27a137.97 ± 6.79h
Root >3.5 kDa103.40 ± 0.01i120.71 ± 3.44h7.05 ± 0.48ab106.66 ± 7.05f
Root Water 72.53 ± 0.02fg184.79 ± 11.05i5.42 ± 0.06a138.45 ± 1.62h

For all the values within a column, different letter superscripts mean significant differences ( < 0.05).