Profiling of Phytochemicals in Tissues from Sclerocarya birrea by HPLC-MS and Their Link with Antioxidant Activity
Table 3
Phytochemicals observed in HPLC-MS analysis of various parts of S. birrea.
Peak nnmber
RT (min)
Proposed compound ID
Empirical formula
Calculated
Observed
MS/MS ions
1
1.54
Disaccharide
C12H21
341.1084
341.1075
179.0, 143.1, 101.0
2
2.02
Gallic acid***
C7H5
169.0137
169.0126
125.0
3
2.74
Procyanidin dimer B2
C30H25
577.1346
577.1326
407.1, 289.0, 169.0, 125.0
4**
2.96
Dihydroxy benzoic acid pentoside
C12H13
285.0610
285.0605
152.1/153.1, 108.1/109.1
5
3.48
Monogalloylated procyanidin dimer B (P2G1)
C37H29
729.1456
729.1442
577.1, 407.1, 289.1, 169.0
6
3.88
Benzyl alcohol hexoside-pentoside
C18H25
401.1448
401.1454
301.0, 269.1, 161.1, 101.0
7
4.57
Hexose derivative
C20H31
431.1917
431.1924
269, 153.1
8
4.66
Digalloylated procyanidin B (P2G2)
C44H33
881.1565
881.1535
729.2, 559.6, 407.1, 169.0
9
5.60
Megastigmane hexoside
C26H35
539.2129
539.2111
491.2, 329.2, 165.1
10
6.28
Megastigmane hexoside
C26H35
539.2129
539.2123
491.2, 329.2, 165.1
11
7.34
Epicatechin 3-O-gallate (P1G1)***
C22H17
441.0822
441.083
289.1, 169.0
12*
8.20
Quercetin 3-O-β-D-(6′′-galloyl)glucopyranoside
C28H23
615.0986
615.0982
463.2, 301.1
13*
8.57
Quercetin 3-O-β-D-(6′′-galloyl)galactopyranoside
C28H23
615.0986
615.0982
463.2, 301.1
14*
9.37
Myricetin 3-O-α-L-rhamnopyranoside
C21H19
463.0877
463.0877
301.1
15*
9.63
Quercetin 3-O-β-D-glucopyranoside***
C21H19
463.0877
463.0887
301.1
16**
10.11
Quercetin 3-O-arabinoside***
C20H17
433.0771
433.0776
301.1
17*
10.53
Kaempferol 3-O-β-D-(6′′-galloyl)glucopyranoside
C28H23
599.1037
599.1041
447.1, 313.1, 285.1, 169.0
18*
11.03
Quercetin 3-O-α-L-rhamnopyranoside***
C21H19
447.0928
447.0941
301.1/300.0, 285.1/284.1
19*
11.36
Quercetin 3-O-α-(5′′-galloyl)arabinofuranoside
C27H21
585.0881
585.0876
433.1, 301.1
20**
11.60
Kaempferol pentoside
C20H17
417.0822
417.0838
285.1/284.0
21*
12.36
Kaempferol 3-O-α-L-rhamnopyranoside
C21H19
431.0978
431.0975
285.1
22**
12.61
Hydroxy benzoyl kaempferol-O-hexoside
C28H23
567.1139
567.1148
447.1, 285.0, 255.0, 137.0
23
12.99
Galloyl hexoside derivative
C26H35
523.2179
523.2172
361.2, 313.1, 169.0
24
2.38
Pentoside derivative
C20H29
461.1659
461.1653
329.1, 191.1, 179.1, 149.1
25
2.79
Catechin***
C15H13
289.0712
289.0700
26
3.12
Galloylepicatechin-epigallocatechin-3-O-gallate
C44H33
897.1514
897.1510
449.1, 407.1, 289.1, 177.0, 125.1
27
3.35
Epigallocatechin 3-O-gallate***
C22H17
457.0771
457.0763
305.1
28
3.75
Epicatechin***
C15H13
289.0712
289.0700
29
4.95
Trigalloylated procyanidin trimer (P3G3)
C66H50
1321.2309
1321.237
1169.3, 1017.3, 881.1, 577.1, 407.0, 169.0
30
5.47
Unknown
C21H19
543.0622
543.0618
31
8.04
Epicatechin-O-gallate (P1G1)
C22H17
441.0822
441.083
289.1, 169.0
32
9.37
Unknown
C13H7
291.0141
291.015
33
10.49
Digalloylated procyanidin dimer B (P2G2)
C44H33
881.1565
881.1577
729.2, 559.6, 407.1, 169.0
34
10.84
Unknown
C16H23
439.1088
439.1078
35
14.67
Unknown
C19H17
357.0975
357.084
36
3.56
Galloyl derivative
C20H21
453.1033
453.104
313.0, 169.0
Identified based on previous report by Braca et al. 2003 [13]. Previously unreported in Sclerocarya birrea. Identification by comparison with standards.