Research Article
Effects of Media Formulation on the Growth and Morphology of Ectomycorrhizae and Their Association with Host Plant
Table 1
Composition of culture media used in this study.
| | Elements | Compounds | MMNa | L-MMNb | G-MMNc | PDA |
| | Macroelements (mg/L) | KH2PO4 | 500.00 | 500.00 | 500.00 | | | (NH4)2SO4 | | 250.00 | | | | (NH4)2HPO4 | 250.00 | | 250.00 | | | MgSO4·7H2O | 150.00 | 150.00 | 150.00 | | | CaCl2·2H2O | 50.00 | 50.00 | 50.00 | | | NaCl | 25.00 | 25.00 | 25.00 | | | Microelements (mg/L) | FeCl3·6H2O | 12.00 | 12.00 | 12.00 | | | H3BO3 | | 15.458 | 15.458 | | | MnSO4·H2O | | 9.295 | 9.295 | | | CuSO4·5H2O | | 1.310 | 1.310 | | | ZnSO4·7H2O | | 5.750 | 5.750 | | | Na2MnO4·2H2O | | 0.003 | 0.003 | | | Vitamins (mg/L) | Thiamine HCl | 1.00 | 10.00 | 0.100 | | | Myo-Inositol | | 100.00 | | | | Nicotinic acid | | 1.00 | | | | Pyridoxine HCl | | 1.00 | | | | Potato (g/L) | | | | | 4 | | Carbohydrate source (g/L) | Dextrose | | | | 20 | | Glucose | 2.5 | 5.0 | 5.0 | | | Malt extract | 10.0 | | 3.0 | | | Solidification agent (g/L) | | 9.0 | 9.0 | 9.0 | 15 |
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Adapted modified media composition based on the media formulation used by Langer et al. [18].
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