Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Codeine Phosphate and Chlorpheniramine Maleate from Their Combined Liquid Dosage Form
Table 3
Optimized chromatographic conditions.
Parameter
Optimized condition
Chromatograph
Shimadzu HPLC with UV detector
Column
Phenomenex C18 column (250 × 4.6 mm, 5 )
Mobile phase
1% o-phosphoric acid in water, pH adjusted to 3.0 : acetonitrile : methanol (78 : 10 : 12)