Development and Validation of an RP-HPLC Method for Estimation of Chlorpheniramine Maleate, Ibuprofen, and Phenylephrine Hydrochloride in Combined Pharmaceutical Dosage Form
Table 1
Validation parameters for CPM, IBU, and PHE.
Parameters
CPM
IBU
PHE
Linearity (range) (µg/mL)
0.5–2.5
25–125
1.25–6.25
Retention time (min)
4.2
13.6
2.7
Detection limit (µg/mL)
0.0321
0.1198
0.0679
Quantitation limit (µg/mL)
0.5
25
1.25
Accuracy (%)
99.44–101.61
99.39–101.79
99.66–101.83
Precision (RSD%)a
Intraday precision ()
0.44–1.28
0.10–0.23
0.38–0.56
Interday precision ()
0.98–1.46
0.66–1.33
0.66–1.53
Instrument precision (RSD%)a ()
1.38
0.57
0.12
RSD is relative standard deviation and “n” is number of determinations.