Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of <i>Tabernaemontana heyneana</i> by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr class="thead"><td align="left" rowspan="2">Levels</td><td align="center">A</td><td align="center">B</td><td align="center">C</td><td align="center">D</td></tr><tr class="thead"><td align="center">Temp. (°C)</td><td align="center">Ext. tim. (hrs)</td><td align="center">Material ratio (g : mL)</td><td align="center">No. of ext. cycles</td></tr><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr><td align="left">1</td><td align="center">60</td><td align="center">1</td><td align="center">1 : 10</td><td align="center">1</td></tr><tr><td align="left">2</td><td align="center">70</td><td align="center">2</td><td align="center">1 : 15</td><td align="center">2</td></tr><tr><td align="left">3</td><td align="center">80</td><td align="center">3</td><td align="center">1 : 20</td><td align="center">3</td></tr><tr class="table-tr"><td colspan="5"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Different variables for optimal extraction of flavonoids from the flowers of <i>T. heyneana</i> (ethyl acetate as extraction solvent, 3<sup>4</sup>-L<sub>9</sub> design).

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Table 2

Table 2: Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of <i>Tabernaemontana heyneana</i> by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry 

Table 2 | Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of Tabernaemontana heyneana by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry 