Research Article
Analysis of the REJ Module of Polycystin-1 Using Molecular Modeling and Force-Spectroscopy Techniques
Table 1
REJ constructs used for SMFS experiments.
| Protein construct | Amino acid (human PC1) Genbank no. L33243 | Expression system | Remarks | Expression vector |
| REJd1 | 2151–2256 | Insect cell Sf9 | Very low expression/insoluble | pFastBac | REJd4 | 2468–2575 | Insect cell Sf9 | Very low expression/insoluble | pFastBac | REJd1-4 | 2151–2575 | Insect cell Sf9 | Very low expression/insoluble | pVL1392 | MBP-REJd1-I27 | 2151–2256 | E. coli | Good expression/soluble | p202 | MBP-REJd1,2-I27 | 2151–2375 | E. coli | Good expression/soluble | p202 | MBP-REJd3,4-I27 | 2380–2575 | E. coli | Very low expression/insoluble | p202 | (I27)3-REJd3,4-(I27)2 | 2380–2575 | E. coli/Sf9 | Good expression/soluble | pRSETA/pVL1392 | (I27)3-REJd4-(I27)2 | 2468–2575 | E. coli/Sf9 | Good expression/soluble | pRSETA/pVL1392 |
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MBP: maltose-binding protein; I27: titin domain I27.
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