Review Article
Critical Factors Affecting the Success of Cloning, Expression, and Mass Production of Enzymes by Recombinant E. coli
Table 1
Some enzymes which are produced in bulk quantities and their industrial applications.
| | Enzymes | Industrial applications |
| | Protease | (i) Inclusion in detergent preparations | | (ii) Cheese making | | (iii) Brewing/backing industries | | (iv) Meat/leather industries | | (v) Animal/human digestive aids. |
| | Amylase | (i) Starch processing industry | | (ii) Fermentation industry |
| Cellulases/
hemicellulases | (i) Brewing industry | | (ii) Fruit juice production | | (iii) Animal feed industry |
| | Pectinases | (i) Fruit juice processing industry |
| | Lipases | (i) Dairy industry
(ii) Vegetable oil industry | | (iii) Leather industry |
| | Glucose isomerase | (i) Production of high-fructose syrups |
| | Lactase | (i) Hydrolysis of milk lactose | | (ii) Digestive aid |
| | Cyclodextrin glycosyltransferase | (i) Production of cyclodextrins for pharmaceuticals and other industries |
| | Penicillin acylase | (i) Production of synthetic penicillin |
| | Carbohydratases | (i) Baking, brewing, confectionary, and fruit industries |
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