International Scholarly Research Notices

Research Article

Downregulation of Caspase-2 Expression in Somitic Cells following Coculture with Chicken Notochord

Table 1

Primer sequences, annealing temperature (AT), the length of amplified products (bp), and number of cycles for different genes.
GenePrimer Sequence (5′→3′)AT (°C)Length (bp)Cycle
GAPDH F: AGTCATCCCTGAGCTGAATG
R: AGGATCAAGTCCACAACACG		5033028
Pax3 F: GCGTCGCAGCAGGACAACCT
R: GCGCTGGGTGGAAACCCTCC		61.521832
BMP4 F: GACCGGCAGGAAGAAAGTCG
R: GCACGCTGCTGCTGAGGTTGAAG		5935232
Bcl2 F: TACCTGCTTACACTTAGGAA
R: ATGACTATGATGCGATGGCA		54.233032
Caspase2 F: GTGGATTCCTGTATTGTAGC
R: ATCCACGCTTGGTGTTGC		49.230235
Pax1 F: GCTGGGTGGTGTCTTCGTGAAC
R: ACTGGTAAAGGGGGTTGTAGGG		5545135
MyoD F: ACTACACGGAATCACCAAATGACC
R: AAGGAATCTGGGCTCCACTGTC		5520035