A Novel Human Membrane Protein Interacting with the Short Fiber of Enteric Adenovirus
Figure 3
Properties of the recombinant His-tagged ParAd41. The His-tagged ParAd41 was expressed in bacteria. After cell lysis in the absence of detergent, the recombinant protein was recovered in the pellet. (a) Solubility of the recombinant protein expressed in bacteria. S—soluble fraction, P—pellet. (b) Oligomeric state. His-ParAd41 purified under denaturing conditions was analyzed by SDS-PAGE. Left panel—staining with CBB, right panel—Western blot with anti-ParAd41 antibody. (c) His-ParAd41 interaction with lipids (see Section 2). LL5a—positive control protein.
