Intracellular localization of ParAd41. (a) Endogenous ParAd41 in HeLa cells analyzed by immunofluorescence microscopy. Fixed cells were incubated with anti-ParAd41 antibody (red signal) and counterstained with the nuclear dye Hoechst (upper panel). (b) Transfected and fixed HeLa cells were incubated simultaneously with anti-ParAd41 (red signal) and anti-lamin B (green signal) and analyzed by confocal microscopy. (c) Transfected Caco-2 cells (undifferentiated: upper panel and differentiated: lower panel) were fixed, incubated with anti-ParAd41 and anti-tubulin or anti-COP antibodies (green signals), and analyzed by confocal microscopy.