Determination of Infectious Bovine Viral Diarrhea Virus in Bovine Lung Lavages by a Combination of Virus Propagation in Cell Culture and Quantitative Real-Time PCR
Figure 3
BVDV titer estimation in lung lavage inoculated MDBK cells. 7 and 14 dpi cells were released by trypsin, analyzed by RT-PCR and BVDV was quantified according to the calibration curve shown in Figure 1(a). Raw data, including standard deviations (SD) and Ct values of the internal control, are presented in Supplementary Table S5.
