Determination of Infectious Bovine Viral Diarrhea Virus in Bovine Lung Lavages by a Combination of Virus Propagation in Cell Culture and Quantitative Real-Time PCR
Figure 4
BVDV detection in lung lavage inoculated MDBK cells by IFT with anti-BVDV FITC-coupled antibodies. Cell cultures were inoculated with lung lavages 1104, 1108, and 1111 and growth on chamber slides was initiated 7 and 14 dpi, respectively. Microscopic examination was performed with an Eclipse E400 fluorescence microscope (Nikon, Germany) at 100–200x magnification under fluorescent light. Photographs were taken using the AxioCam MRC and analyzed with AxioVision Rel 4.7 (Zeiss, Jena, Germany). Scale bar 100 μm.
