Research Article
Fecal Collection and Stabilization Methods for Improved Fecal DNA Test for Colorectal Cancer in a Screening Setting
Table 1
Summary of stool samples preservation.
| | Buffer | Hold time* | Number of samples |
| | A Refrigerator storage | Fresh (0 H) | 7 | | 24 H | 7 | | 36 H | 7 | | 72 H | 7 | | B Buffer EDTA 100 mM | Fresh (0 H) | 7 | | 24 H | 7 | | 36 H | 7 | | 72 H | 7 | | 120 H | 7 | | C Buffer Genefec | Fresh (0 H) | 7 | | 24 H | 7 | | 36 H | 7 | | 72 H | 7 | | 120 H | 7 | | D Buffer EDTA 20 mM | Fresh (0 H) | 7 | | 24 H | 7 | | 36 H | 7 | | 72 H | 7 | | 120 H | 7 | | E RNAlater RNA Stabilization Reagent | Fresh (0 H) | 7 | | 24 H | 7 | | 36 H | 7 | | 72 H | 7 | | 120 H | 7 | | F Cytolyte | Fresh (0 H) | 7 | | 24 H | 7 | | 36 H | 7 | | 72 H | 7 | | 120 H | 7 | | G Buffer -Wasserman | Fresh (0 H) | 7 | | 24 H | 7 | | 36 H | 7 | | 72 H | 7 | | 120 H | 7 |
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The old time is the amount of time the sample is held in preservative prior to transfer to −80°C freezer.
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