Research Article
Fecal Collection and Stabilization Methods for Improved Fecal DNA Test for Colorectal Cancer in a Screening Setting
Table 2
PCR primer set of genes analyzed to assess DNA amplificability and integrity.
| Region | Forward primer | Reverse primer | Product size (bp) | Annealing temperature (°C) |
| KRAS | ACTGAATATCTTGTGGTAGTTGGAGCT | TCAAAGAATGGTCCTGGACC | 157 | 54 | TP53 ex 6 | CTGGAGAGACGACAGGGCTG | GACAACCACCCTTAACCCCTC | 219 | 60 | TP53 ex 7 | CTTGCCACAGGTCTCCCCAA | TCAGCGGCAAGCAGAGGCTG | 233 | 60 | TP53 ex 8 | CGCCCGCCGCGCCCCGCGCCCGTCCCG CCGCCCCCGCCCGACTGCCTCTTGCTTCTC TTTTCC | AATCTGAGGCATAACTGCACCC | 271 | 62 | NRAS | GTCACTTTAAGAACCAAATGG | GAGAGACAGGATCAGGTCA | 288 | 54 | NRAS | CTTGGCAATAGCATTGTATTC | GATTCAGAACACAAAGATCATC | 315 | 54 | TP53 ex 5 | GCTGCCGTGTTCCAGTTGC | TCAGTGAGGAATCAGAGGCC | 330 | 60 | KRAS | GTTGAGTTGTATATAACACC | ATTATATGCATGGCATTAGC | 343 | 54 |
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