Target Primers Sequence from 5′ to
Annealing temperature Reference Akkermansia muciniphila AM1 CAGCACGTGAAGGTGGGGAC 50°C
[18 ] AM2 CCTTGCGGTTGGCTTCAGAT Bifidobacterium genusBifido5′ GAT TCT GGC TCA GGA TGA ACG C 60°C [19 , 20 ] Bifido3′ CTG ATA GGA CGC GAC CCC AT Bifidobacterium adolescentis Ado-Ang5′ GGA TCG GCT GGA GCT TGC TCC G 63°C
[21 ] Ado3′ CCC CGA AGG CTT GCT CCC AGT Bifidobacterium bifidum Bifidum5′ TGA CCG ACC TGC CCC ATG CT 61°C
[21 ] Bifidum3′ CCC ATC CCA CGC CGA TAG AAT Bifidobacterium breve Breve5′ AAT GCC GGA TGC TCC ATC ACA C 62°C
[21 ] Breve3′ GCC TTG CTC CCT AAC AAA AGA GG Bifidobacterium catenulatum groupCaten5′ GCC GGA TGC TCC GAC TCC T 64°C
[21 ] Caten3′ ACC CGA AGG CTT GCT CCC GAT Bifidobacterium longum groupLongum5′ TTC CAG TTG ATC GCA TGG TCT TCT 65°C
[21 ] Longum3′ GGC TAC CCG TCG AAG CCA CG Clostridium coccoides groupg-Ccoc-F AAA TGA CGG TAC CTG ACT AA 53°C
[22 ] g-Ccoc-R CTT TGA GTT TCA TTC TTG CGA A Clostridium difficile Cdif-F TTG AGC GAT TTA CTT CGG TAA AGA 62°C
[23 ] Cdif-R TGT ACT GGC TCA CCT TTG ATA TTY A Clostridium leptum subgroupsg-Clept-F GCA CAA GCA GTG GAG T 60°C
[24 ] sg-Clept-R3 CTT CCT CCG TTT TGT ACC Clostridium perfringens CPerf165F CGC ATA AYb G TTG AAA GAT GG 64°C
[25 ] CPerf269R CCT TGG TAG GCC GTT ACC C Staphylococcus aureus nuc geneNUC1 GCG ATT GAT GGT GAT ACG GTT 55°C
[26 ] NUC2 AGC CAA GCC TTG ACG AAC TAA AGC
