Research Article
On the Combined Application of Iatroscan TLC-FID and GC-FID to Identify Total, Neutral, and Polar Lipids and Their Fatty Acids Extracted from Foods
Table 1
Retention time, calibration data, and reproducibility for neutral and polar lipid standards.
| | Solvent system | Lipid standards | Retention time | Regression equation | Correlation coefficients () | CV% of intraday variability | CV% of interday variability |
| Solvent A
(n-H : DE : FA) (60 : 15 : 1.5 by vol.) | HC | 0.02–0.03 | | 0.9994 | 1.28 | 1.69 | Sterol esters
(wax esters) | 0.04–0.06 | | 1 | 1.34 | 1.76 | | TG | 0.07–0.10 | | 0.9984 | 2.33 | 1.83 | | FFA | 0.11–0.12 | | 0.9926 | 1.57 | 2.21 | | 1,3-DG | 0.16–0.18 | | 0.9987 | 1.62 | 2.49 | | Sterol | 0.20–0.24 | | 1 | 2.28 | 3.46 | | 1,2-DG | 0.31–0.34 | | 1 | 1.29 | 1.87 | | MG | 0.36–0.39 | | 0.9993 | 1.56 | 2.17 | | PL | 0.42–0.45 | — | — | — | — |
| Solvent B
(C : M : W)
(50 : 20 : 2, by vol.) | NL | 0.02–0.08 | — | — | — | — | | PE | 0.09–0.11 | | 0.9987 | 1.74 | 2.86 | | PI | 0.11–0.14 | | 0.9997 | 2.64 | 3.35 | | PS | 0.15–0.18 | | 0.9997 | 2.47 | 3.68 | | l-PE | 0.19–0.20 | | 0.9995 | 1.92 | 3.04 | | PnL | 0.21–0.23 | — | — | — | — | | PC | 0.24–0.30 | | 0.9997 | 1.40 | 2.48 | | Sphm | 0.33–0.35 | | 0.9994 | 1.76 | 2.55 | | l-PC | 0.37–0.40 | | 0.9998 | 1.63 | 2.26 |
|
|
Development distance/times of development: 10 cm (1 time).
|
