Effects of feeding diets differing in sulfur amino acid levels on rpS6 phosphorylation status and eIF1α phosphorylation status in liver of rats. Values are means ± SEM for 4 rats. Values represented by bars not labeled with the same letter are significantly different at by ANOVA and Tukey’s comparison; values for phospho-eIF2α and eIF2αP/total eIF2α were transformed to square roots prior to statistical analysis. Representative western blots are shown; equal amounts of total soluble protein were loaded per lane with the order of samples the same as for the bar graphs. The amounts of the indicated proteins were normalized by actin. To avoid the dependence of ratios on exposure times with the different antibodies, rpS6-P/total rpS6 ratios and eIF2α-P/total eIF2α ratios were calculated as relative ratios after first expressing densities as fold of the control group.