Research Article

Use of a Multiplex PCR for the Detection of Toxin-Encoding Genes netB and tpeL in Strains of Clostridium perfringens

Table 1

Bacterial strains used as positive and negative controls in a multiplex polymerase chain reaction designed for detection of Clostridium perfringens genes netB and tpeL.

Strain ID numberSpeciesnetB tpeL RelevanceSource

C103-99Clostridium perfringens ++Positive controlNecrotic enteritis
JGS-1870Clostridium perfringens ++Positive controlNecrotic enteritis
JGS-4140Clostridium perfringens ++Positive controlNecrotic enteritis
232-2Clostridium bifermentans Negative controlPoultry litter
259-14Bacteroides caccae Negative controlPoultry litter
250-4Wolinella sp.Negative controlPoultry litter